To develop simple, rapid, accurate, precise and reproducible reversed phase high performance liquid chromatographic method has been developed for the estimation of Coumarin in bulk and in tablets. Development of method for Coumarin determination of by RP-HPLC was undertaken using new mobile phase of Methanol: water (70:30 v/v). The eluent was monitored at 300 nm. The optimized conditions developed showed a linear response from 2 to 20 ug/mL with correlation coefficient of 0.9998. The retention time of drug was found to be 3.2 minutes. The limit of detection (LOD) and limit of quantification (LOQ) were 0.025 and 0.037ug/mL. Recovery data were good. Placebo study for specificity and interference of common excipients showed that the method was specific and free from interfering substances. Therefore, the fully validated method developed was sensitive enough to carry out analysis of Coumarin in tablet formulations with regard to its run time, simplicity of sample preparation and accuracy.
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